Edinburg Genome Foundry
less than a minute
https://edinburgh-genome-foundry.github.io/
This group build the tool: DNA Feature Viewer.
Some other tools are also intersting.
DNA Cauldron
DNA Cauldron (full documentation here) is a generic cloning simulation framework to predict final construct sequences and detect assembly flaws. It aims in particular at automating the simulation and verification of large (and possibly multi-step) DNA assembly batches, with the idea that, if your assembly plan works with Cauldron, you’ll get the same results at the bench.
Great support for Golden Gate assembly (incl. MoClo, EMMA, Phytobrick, etc.), the simulation is as simple as dropping sequence files in a web app.
Good support for Gibson, Biobrick, BASIC, LCR Assembly.
Provide genetic parts in any order, with or without annotations, in reverse or direct sense, linear or circular, single or combinatorial assembly… Cauldron will get it!
Design flaw detection (missing parts, unwanted restriction sites, wrong overhang designs, etc.).
If your assembly needs connector parts, Cauldron can select them for you!
Import batch assembly plans from spreadsheets, including hierarchical (=multi-step) plans.
Comprehensive reports with constructs sequences, fragment sequences, summary spreadsheets.
Export multi-file reports in folders, zip files, or in-memory zip files (for use on servers).
Web UI
They also built web UIs for their tools. for example: